General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR152 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR152 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR152 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx cAMP Hunter™ Human Apelin (APJ Receptor) Gi cAMP Cell-Based Assay Kit (CHO-K1)
The cAMP Hunter™ Human Apelin (APJ Receptor) Gi Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure AGTRL1 (GPCR) activity via decreased levels of intracellular cAMP. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human PPYR1 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human PPYR1 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure PPYR1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical CalcIum asy KIt 192Wel
Cayman's Calcium Assay provides a quick, reliable method for determining total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates. The assay utilizes an optimized variant of the well-established o-Cresolphthalein-calcium reaction in which a vivid purple complex that absorbs between 560 and 590 nm is formed in the presence of calcium.
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0500E2CP19L)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress CMKLR1 Activated GPCR Internalization Assay, 10pl Kit. (93-0720E3CP22L)
The PathHunter CMKLR1 Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of CMKLR1 via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged CMKLR1. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting CMKLR1-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery U-PLEX Human Nectin-4 ASY
The Meso Scale Discovery Human Nectin-4 Singleplex Assay Kit is a singleplex immunoassay that measures Nectin-4 levels in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity wide dynamic range and low sample volume requirements The assay requires 50 L of sample and can be read on Meso Scale Discovery SECTOR Imagers The assay has a typical lower limit of detection of 0 03 ng/mL and an upper limit of quantification of 2500 ng/mL This assay kit includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer calibrator and control
ILEX LIFE SCIENCES LLC QuickZyme Human MMP-7 Activity Assay Kit, 96 Assays, 96-well plate format, immunoassay for active or total matrix metalloproteinase 7 (conditioned culture medium, serum, urine, tissue homogenates samples)
The QuickZyme Human MMP-7 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure both endogenous active MMP-7 and total active MMP-7 (following activation of pro-MMP-7 on the plate with APMA). It can be used for the measurement of MMP-7 activity in various biological samples, such as conditioned culture media, tissue homogenates, serum, plasma and urine. This 96-well plate format assay is based on the QuickZyme technology, using a modified pro-enzyme as a substrate, which upon activation is able to release color from a chromogenic peptide substrate. This multiplication step provides a unique assay sensitivity. ASSAY RANGE: 0.004 ¿ 16 ng/ml. SENSITIVITY: 30 pg/ml (3 h incubation); 4 pg/ml (25 h incubation). SYNONYMS: Matrix metalloproteinase-7, MMP7, Matrilysin, Matrilysin-1, Matrin, Pump-1 protease, Uterine metalloproteinase.
10X Genomics Inc GEM-X Flex Gene Expression Human n-plex, 64 samples
GEM-X Flex Gene Expression Human n-plex, 64 samples
Carterra SPR Camp - Andover MA CEC
SPR Camp - Andover, MA CEC
Cayman Chemical NonradIoactIve IodID asy K 96
A B2-AR agonist selectively binds to B1- over B2-ARs (Kds 2.9 and 113 nM respectively) as well as B3-adrenergic B2 bradykinin NK1 and NK2 receptors up to 3 M induces cAMP accumulation in cultured human bronchial epithelial cells relaxes guinea pig tracheal strips precontracted with carbamoylcholine ovalbumin or histamine (pD2s 8.4 9.5 and 9.5 respectively) reverses histamine- and ovalbumin-induced bronchoconstriction in guinea pigs (ED50s 1 and 40 nmol/kg respectively)
Eurofins Discoverx PathHunter Human OPRM1 (Opioid mu) β-Arrestin-1 Cell-Based Assay Kit (U2OS)
The PathHunter Human OPRM1 (Opioid mu) β-Arrestin-1 Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure OPRM1 (GPCR) activity via recruitment of β-Arrestin-1. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx InCELL Hunter eXpress CLK1 Kinase Binding Assay, 2pl Kit. (96-0059E1CP0M)
The InCELL Hunter eXpress CLK1 Kinase Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with kinases. This kit assesses compound-engagement-based stabilization of CLK1 via detection of CLK1 levels. This product uses EFC technology. The cell line included overexpresses CLK1 target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human Visfatin ASY
The Meso Scale Discovery R-PLEX Human Visfatin Assay measures visfatin in human serum plasma and cell culture supernatants using electrochemiluminescence (ECL) detection It offers high sensitivity a 5-log dynamic range and low sample volume needs Fully quantitative with an 8-point calibration curve it is lyophilized for stability The R-PLEX platform allows multiplexing with other biomarkers This assay provides robust data for research in obesity diabetes metabolic disease and immunology
TRIBIOSCIENCE INC Glutamate Quantification Colorimetric Assay 100 Tests
Tribioscience Glutamate Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction converting chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of glutamate in the samples. The kit provides the easiest and most accurate approach to measuring glutamate from a variety of samples. Applications - Specifically detect glutamate from cell and biological tissues.